Human TLR7 antibody

TLR7 is one of toll-like receptors which are type I transmembrane proteins involved in innate immunity by recognizing microbial conserved structures. These proteins are characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain, and act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. Human TLR7 (hTLR7) is predominantly expressed in lung, placenta and spleen and hTLR7, 8 genes are located on the sex chromosome X. hTLR7 may play an important role in detecting and combating viral infections.

Product Specifications

Product Name Alternative

Toll-like receptor 7, PRO285, UNQ248, tlr 7

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TLR7 (451-500aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

4F4

Conjugation

Unconjugated

Applications

ELISA, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TLR7, ATR0601-10 µg, ATR0601-20 µg, ATR0601-50 µg, ATR0601-100 µg, ATR0601-250 µg, ATR0601-500 µg, ATR0601-1 mg, ATR0601-10, ATR0601-20, ATR0601-50, ATR0601-100, ATR0601-250, ATR0601-500, ATR0601-1

References & Citations

Lee J., et al. (2003) Proc. Natl. Acad. Sci. USA. 100, 6646-6651.; ; Crozat K., et al. (2004) Proc. Natl. Acad. Sci. USA. 101, 6835-6836.; ; Muzio M., et al. (1998) J. Exp. Med. 187, 2097-2101.; ; Horng T., et al. (2002) Nature 420, 329-333.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_057646

Uniprot Accession Number

Q9NYK1

FACS Description

Flow cytometry analysis of TLR7 in THP-1 cells. The cell was stained with ATR0601 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of TLR7 in THP-1 cells. The cell was stained with ATR0601 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .