Human Tollip antibody

Toll interacting protein, also known as Tollip, is an inhibitory adaptor protein within the signaling pathway of IL-1 and Toll-like receptors (TLR) . The Toll-like receptors are essential transmembrane signaling receptors of the innate immune system that alert the host to the presence of a microbial invader. Tollip recruits IRAk1 to the IL-1 receptor complex and inhibits IRAk1 phosphorylation and kinase activity.

Product Specifications

Product Name Alternative

Toll interacting protein, IL-1RAcPIP

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Tollip (61-274aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

K1B17

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Tollip, ATO0918-10 µg, ATO0918-20 µg, ATO0918-50 µg, ATO0918-100 µg, ATO0918-250 µg, ATO0918-500 µg, ATO0918-1 mg, ATO0918-10, ATO0918-20, ATO0918-50, ATO0918-100, ATO0918-250, ATO0918-500, ATO0918-1

References & Citations

Ciarrocchi A. et al., (2009) Published online. 4 (2) :e4404.; ; Didierlaurent A. et al., (2006) Mol Cell Biol. 26 (3) :735-742.; ; Opal S.M. et al., (2002) Crit Care. 6 (2) :125-136.; ; Burns K. et al., (2000) Nat Cell Biol. 2 (6) :346-351.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_061882

Uniprot Accession Number

Q9H0E2

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tollip antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 2.: HeLa cell lysate;

IF Description

ICC/IF analysis of Tollip in THP-1 cells. The cell was stained with ATO0918 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .