What is the catalog number for Recombinant human CD23/FCER2 protein?

The catalog number for Recombinant human CD23/FCER2 protein is ATGP4162-250.

What sizes are available for Recombinant human CD23/FCER2 protein?

Recombinant human CD23/FCER2 protein is available in the following size(s): 250 µg.

Is Recombinant human CD23/FCER2 protein in stock?

Recombinant human CD23/FCER2 protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human CD23/FCER2 protein

CD23/FCER2, also known as low affinity immunoglobulin epsilon Fc receptor isoform a, is a member of subgroup II of the C-type (Ca++-dependent) lectin superfamily. It is a low affinity receptor for B cell specific antigen and IgE. Unlike many of the antibody receptors, CD23 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. It plays an essential role in the growth and differentiation of B cells, and in the regulation of IgE production. This protein also exists in a soluble secretion form and functions as a powerful cleavage-promoting growth factor. Increased levels of soluble CD23/FCER2 lead to the recruitment of unaffected B cells in the presentation of antigen peptides to allergen-specific B cells. Recombinant human CD23/FCER2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human IgE is coated at 5 µg/mL (100 ul/well) can bind Human CD23/FCER2. The ED50 range ≤ 15 µg/mL.

Product Name Alternative

Low affinity immunoglobulin epsilon Fc receptor, BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, CD23, CD23A, CLEC4J, FCE2, IGEBF, FCER2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human IgE. The ED50 range ≤ 15 µg/mL.

Molecular Weight

32 kDa (283aa)

Additionnal Information

ATGP4162-10 µg, ATGP4162-20 µg, ATGP4162-50 µg, ATGP4162-100 µg, ATGP4162-250 µg, ATGP4162-500 µg, ATGP4162-1 mg, ATGP4162-010, ATGP4162-020, ATGP4162-050, ATGP4162-100, ATGP4162-250, ATGP4162-500, ATGP4162-01M, ATGP3705-10 µg, ATGP3705-20 µg, ATGP3705-50 µg, ATGP3705-100 µg, ATGP3705-250 µg, ATGP3705-500 µg, ATGP3705-1 mg, ATGP3705-010, ATGP3705-020, ATGP3705-050, ATGP3705-100, ATGP3705-250, ATGP3705-500, ATGP3705-01M

References & Citations

Heyman, B., et al. (2000) Annu. Rev. Immunol. 18:709-737.; ; Bajorath, J., et al. (1996) Protein Sci. 5:240-247.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001993.2

Uniprot Accession Number

P06734

Species

Human

N Terminal Sequence

Ala

AA Sequence

DTTQSLK QLEERAARNV SQVSKNLESH HGDQMAQKSQ STQISQELEE LRAEQQRLKS QDLELSWNLN GLQADLSSFK SQELNERNEA SDLLERLREE VTKLRMELQV SSGFVCNTCP EKWINFQRKC YYFGKGTKQW VHARYACDDM EGQLVSIHSP EEQDFLTKHA SHTGSWIGLR NLDLKGEFIW VDGSHVDYSN WAPGEPTSRS QGEDCVMMRG SGRWNDAFCD RKLGAWVCDR LATCTPPASE GSAESMGPDS RPDPDGRLPT PSAPLHS