Recombinant human CD23/FCER2 protein
CD23/FCER2, also known as low affinity immunoglobulin epsilon Fc receptor isoform a, is a member of subgroup II of the C-type (Ca++-dependent) lectin superfamily. It is a low affinity receptor for B cell specific antigen and IgE. Unlike many of the antibody receptors, CD23 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. It plays an essential role in the growth and differentiation of B cells, and in the regulation of IgE production. This protein also exists in a soluble secretion form and functions as a powerful cleavage-promoting growth factor. Increased levels of soluble CD23/FCER2 lead to the recruitment of unaffected B cells in the presentation of antigen peptides to allergen-specific B cells. Recombinant human CD23/FCER2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IgE is coated at 5 µg/mL (100 ul/well) can bind Human CD23/FCER2. The ED50 range ≤ 15 µg/mL.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human IgE. The ED50 range ≤ 15 µg/mL.
Molecular Weight
32 kDa (283aa)
Additionnal Information
References & Citations
Heyman, B., et al. (2000) Annu. Rev. Immunol. 18:709-737.; ; Bajorath, J., et al. (1996) Protein Sci. 5:240-247.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_001993.2
Uniprot Accession Number
P06734
Species
Human
N Terminal Sequence
Ala
AA Sequence
Frequently Asked Questions
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