Recombinant mouse VEGFR1/Flt-1 protein

VEGFR1 (Vascular Endothelial Growth Factor Receptor 1), also known as FLT-1 (Fms-like Tyrosine Kinase 1), belongs to the class III subfamily of receptor tyrosine kinases. While VEGFR1, VEGFR2, and VEGFR3 are mainly expressed on endothelial cells, VEGFR1 is uniquely expressed on macrophages and primarily plays inhibitory roles. Inhibitors of VEGFR are used in cancer treatment. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB, and PGF, playing essential roles in the development of embryonic vasculature, regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 binds VEGF with higher affinity than VEGFR2 but exhibits weaker kinase activity. The VEGF-kinase ligand/receptor signaling system is crucial for vascular development and the regulation of vascular permeability. Recombinant mouse VEGFR1/Flt-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse VEGFR1/Flt-1 inhibit proliferation of the HUVEC human umbilical vein endothelial cells in the presence of Human VEGF165. The ED50 range ≤ 50 ng/mL.

Product Name Alternative

VEGFR1, VEGF R1, VEGFR-1, FLT-1, FLT1, FLT, sFlt1, Emrk2, FRT, Vascular endothelial growth factor receptor 1 isoform 1, Embryonic receptor kinase 2, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor, fms-related tyrosine kinase 1

Expression System

HEK293

Antigen Species

Mouse

Tag

HIgG-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Bioactivity

Measured by its ability to inhibit proliferation using HUVEC human umbilical vein endothelial cells in the presence of Human VEGF165. The ED50 range ≤ 50 ng/mL.

Molecular Weight

108.9kDa (970aa)

Additionnal Information

ATGP4160-10 µg, ATGP4160-20 µg, ATGP4160-50 µg, ATGP4160-100 µg, ATGP4160-250 µg, ATGP4160-500 µg, ATGP4160-1 mg, ATGP4160-010, ATGP4160-020, ATGP4160-050, ATGP4160-100, ATGP4160-250, ATGP4160-500, ATGP4160-01M

References & Citations

Peters, K.G. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8915-8919.; ; Kendall R.L., et al., (1993), Natl. Acad. Sci. U.S.A. 90:10705-10709.; ; Herley M.T., et al., (1999), Biochem. Biophys. Res. Commun. 262:731-738.; ; Kendall R.L., et al., (1993), Natl. Acad. Sci. U.S.A. 90:10705-10709.