Recombinant human LILRA3/CD85e protein

LILRA3, also known as CD85 antigen-like family member E (CD85e), immunoglobulin-like transcript 6 (ILT-6), and leukocyte immunoglobulin-like receptor 4 (LIR-4), acts as a soluble receptor for class I MHC antigens. It binds both classical and non-classical HLA class I molecules, albeit with reduced affinities compared to LILRB1 or LILRB2. Additionally, LILRA3 exhibits high affinity for monocyte surfaces, effectively suppressing LPS-induced TNF-alpha production. Unlike many of its family, LILRA3 lacks a transmembrane domain. The function of LILRA3 is currently unknown. However, it is highly homologous to other LILR genes and can bind human leukocyte antigen (HLA) class I. Therefore, if secreted, the LILRA3 might impair interactions of membrane-bound LILRs with their HLA ligands, thus modulating immune reactions and influencing susceptibility to disease. Recombinant human LILRA3/CD85e, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.