Recombinant human TRANCE/RANK L/TNFSF11 protein

TNFSF11, also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. TNFSF11 also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. TNFSF11 is important in bone metabolism. This natural and necessary surface-bound molecule (also known as CD254) found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption. Recombinant human TNFSF11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Human TRANCE stimulates osteoclast differentiation in the RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤ 25 ng/mL.

Product Name Alternative

Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS – PAGE

Bioactivity

Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤ 25 ng/mL.

Molecular Weight

22.3 kDa (199aa) confirmed by MALDI-TOF

Additionnal Information

TRANCE , TNFSF11, RANK L, Tumor necrosis factor ligand superfamily member 11, OPGL, RANKL, ATGP4157-10 µg, ATGP4157-20 µg, ATGP4157-50 µg, ATGP4157-100 µg, ATGP4157-250 µg, ATGP4157-500 µg, ATGP4157-1 mg, ATGP4157-10, ATGP4157-20, ATGP4157-50, ATGP4157-100, ATGP4157-250, ATGP4157-500, ATGP4157-1

References & Citations

Lam J., et al. (2001) J. Clin. Invest. 108:971-979 Ito S., et al. (2002) J. Biol. Chem. 277:6631-6636

Storage Conditions

Can be stored at +4°C short term (1-2 weeks) . For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_003692.1

Uniprot Accession Number

O14788

Species

Human

AA Sequence

IRAEKAMVD GSWLDLAKRS KLEAQPFAHL TINATDIPSG SHKVSLSSWY HDRGWAKISN MTFSNGKLIV NQDGFYYLYA NICFRHHETS GDLATEYLQL MVYVTKTSIK IPSSHTLMKG GSTKYWSGNS EFHFYSINVG GFFKLRSGEE ISIEVSNPSL LDPDQDATYF GAFKVRDID