Recombinant human NPEPPS protein

NPEPPS, also known as PSA, is a zinc metallopeptidase with broad substrate specificity for several peptides. It hydrolyzes N‑terminal amino acids from its substrates. This protein is expressed in most tissues as a cytoplasmic protein, but a membrane-associated form has been identified in the brain. This protein is involved in proteolytic events which is essential for cell growth and viability. It also may acts as regulator of neuropeptide activity. It is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is found at high levels in the urine when there are kidney problems. It is also known to degrade the tau protein, which accumulates and polymerizes in some neurodegenerative diseases. Recombinant human NPEPPS, fused to His-tag at C-terminus, was expressed in HEK293 and purifed by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 800 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1 pmole of H-Leu-AMC per minute at pH7.0 at 37°C.

Product Name Alternative

Puromycin-sensitive aminopeptidase, PSA, Cytosol alanyl aminopeptidase, AAP-S, MP100, aminopeptidase puromycin sensitive

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Bioactivity

Specific activity is > 800 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1 pmole of H-Leu-AMC per minute at pH7.0 at 37°C.

Molecular Weight

104kDa (925aa)

Additionnal Information

Puromycin-sensitive aminopeptidase, PSA, Cytosol alanyl aminopeptidase, AAP-S, MP100, aminopeptidase puromycin sensitive, ATGP4156-10 µg, ATGP4156-20 µg, ATGP4156-50 µg, ATGP4156-100 µg, ATGP4156-250 µg, ATGP4156-500 µg, ATGP4156-1 mg, ATGP4156-10, ATGP4156-20, ATGP4156-50, ATGP4156-100, ATGP4156-250, ATGP4156-500, ATGP4156-1

References & Citations

Kudo LC, et al. (2011) Hum Mol Genet. 20:1820-1833.; ; Jones RT, et al. (2024) Cancer Res. 84:1699-1718.; ; Lenskaya V, et al. (2024) J Cutan Pathol. 51:419-423.;