Recombinant human GFR alpha-1 protein
GFR alpha-1, also known as GDNF family receptor alpha-1, belongs to the GDNFR family. It is a glycosylphosphatidylinositol (GPI) -linked receptor found on the cell surface, acting as a receptor for both GDNF and NTN and function as neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. It bind GDNF strongly and activate RET tyrosine kinase and is also essential for the development of kidneys and the enteric nervous system. Recombinant human GFR alpha-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human GDNF (CAT# ATGP3942) is coated at 0.5 µg/mL (100 ul/well) can bind Human GFR alpha-1. The ED50 range ≤ 50 ng/mL.
Product Name Alternative
Expression System
HEK293
Antigen Species
Human
Tag
HIgG-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human GDNF (CAT# ATGP3942) . The ED50 range ≤ 50 ng/mL.
Molecular Weight
70.8 kDa (633aa)
Additionnal Information
References & Citations
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_665736.1
Uniprot Accession Number
P56159
Species
Human
N Terminal Sequence
Asp 25
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