Recombinant Human Osteocrin protein

Osteocrin, also known as Musclin, shows a well conserved homology with members of the natriuretic peptide (NP) family. It is a secreted protein that is expressed in muscle and bone. Based on similarities with NPs, Osteocrin could interact with the NP clearance receptors, importantly increasing CNP which has been shown to stimulate endochondral ossification and elongate bones. It is represents a novel, unique vitamin D-regulated bone-specific. Recombinant human Osteocrin, fused to His-tag at C-terminus, was expressed in HEK 293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human NPRC is coated at 2 µg/mL (100 ul/well) can bind Human OSTN. The ED50 range ≤ 20 ng/mL.

Product Name Alternative

Osteocrin, MUSCLIN, OSTN

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human NPRC. The ED50 range ≤ 20 ng/mL.

Molecular Weight

12.5kDa (112aa)

Additionnal Information

ATGP4150-10 µg, ATGP4150-20 µg, ATGP4150-50 µg, ATGP4150-100 µg, ATGP4150-250 µg, ATGP4150-500 µg, ATGP4150-1 mg, ATGP4150, ATGP4150-020, ATGP4150-050, ATGP4150-100, ATGP4150-250, ATGP4150-500, ATGP4150-01M

References & Citations

Thomas, G., et al, (2003) J. Biol. Chem. 278:50563–50571.; ; Nishizawa H., et al, (2004) J Biol Chem. 279:19391-19395.; ; Pierre Moffatt., et al, (2007) J Biol Chem. 282:36454-36462.