Recombinant human Fc gamma RIIB/CD32b protein

Fc gamma RIIB/CD32b, also known as FCGR2B, belongs to the Ig superfamily. It is a low-affinity receptor for the Fc region of immunoglobulins gamma that are complexed or aggregated. It participates in various effector and regulatory functions, such as phagocytosis of immune complexes and modulation of antibody production by B cells. Fc gamma RIIB/CD32b is expressed on B cells, monocytes, dendritic cells, neutrophils, mast cells, and basophils. When Fc gamma RIIB is ligated, it inhibits B cell expansion, plasma cell differentiation, production of autoimmune rheumatoid factors, and down-regulates TLR4 with reduced LPS responsiveness. Recombinant human Fc gamma RIIB/CD32b, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human Fc gamma RIIB/CD32b (CAT# ATGP4149) is coated at 10 µg/mL (100 ul/well) can bind Human IgG1 Fc (CAT# ATGP4094) in a Functional ELISA assay.

Product Name Alternative

Low affinity immunoglobulin gamma Fc region receptor II-b isoform3, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b, CD32, FCGR2B, FCG2, IGFR2, Fc fragment of IgG, low affinity II, low affinity IIb, Low affinity immunoglobulin gamma Fc region receptor II-c, IgG Fc receptor II-c, Fc-gamma RII-c, Fc fragment of IgG receptor IIb, Fc-gamma-RIIc, Fc gamma RIIb, CD32B, FcgammaRIIb, FCGR2, FCGR2C, FcGRIIB, FcRII-c

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human IgG1 Fc (CAT# ATGP4094) .

Molecular Weight

20.1kDa (178aa)

Additionnal Information

ATGP4149-10 µg, ATGP4149-20 µg, ATGP4149-50 µg, ATGP4149-100 µg, ATGP4149-250 µg, ATGP4149-500 µg, ATGP4149-1 mg, ATGP4149-010, ATGP4149-020, ATGP4149-050, ATGP4149-100, ATGP4149-250, ATGP4149-500, ATGP4149-01M, ATGP1822-10 µg, ATGP1822-20 µg, ATGP1822-50 µg, ATGP1822-100 µg, ATGP1822-250 µg, ATGP1822-500 µg, ATGP1822-1 mg, ATGP1822-010, ATGP1822-020, ATGP1822-050, ATGP1822-100, ATGP1822-250, ATGP1822-500, ATGP1822-01M

References & Citations

Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215-221.; ; Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181-220.; ; Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275-290.