Recombinant mouse EphA3 protein

EphA3 is a protein-tyrosine kinase that belongs to the ephrin receptor subfamily. It has an extracellular region with a Cys-rich domain and two fibronectin type III repeats, and a single kinase domain. EPH and other EPH-related receptors are involved in various developmental processes, especially in the nervous system. EphA3 interacts with EFNB2 and EFNA5, and is expressed in the forebrain, retinal axons, some motor neurons in the spinal cord, and the heart during development. It regulates axonal guidance and organ formation. It is also found on some blood and solid tumor cells, and on astrocytes near injured nerve tissue. Recombinant mouse EphA3, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse EphA3 (CAT# ATGP4148) is coated at 2 µg/mL (100 ul/well) can bind Human EFNA5 (CAT# ATGP4147) in a Functional ELISA assay.

Product Name Alternative

Ephrin type-A receptor 3, ephrin type-A receptor 3 isoform1, receptor protein-tyrosine kinase, EPH-like kinase 4, tyrosine-protein kinase TYRO4, tyrosine-protein kinase receptor ETK1, Eph receptor A3, AW492086, Cek4, EK4, End3, ETK1, Hek, Hek4, Mek4, Tyro4

Expression System

HEK293

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human EFNA5 (CAT# ATGP4147) .

Molecular Weight

59.5kDa (527aa)

Additionnal Information

ATGP4148-10 µg, ATGP4148-20 µg, ATGP4148-50 µg, ATGP4148-100 µg, ATGP4148-250 µg, ATGP4148-500 µg, ATGP4148-1 mg, ATGP4148-010, ATGP4148-020, ATGP4148-050, ATGP4148-100, ATGP4148-250, ATGP4148-500, ATGP4148-01M

References & Citations

Kudo, C. et al. (2005) J. Comp. Neurol. 487:255-269.; ; Kilpatrick, T.J. et al. (1996) Mol. Cell. Neurosci. 7:62-74.; ; Stephen, L.J. et al. (2007) Dev. Biol. 302:66-79.; ; Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194-200.