Recombinant human Ephrin-A5 protein

Ephrin-A5, as known as EFNA5, is a member of the ephrin ligand family which binds the members of ephrin receptor subfamily of tyrosine kinases. This protein is expressed with the highest levels in human adult brain, heart, spleen, and ovary and human fetal brain, lung, and kidney. It is also expressed by muscle precursor cells and interacts with ephrin-A4 to restrict their migration to the correct locations during forelimb morphogenesis. Recombinant human Ephrin-A5, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse EphA3 (CAT# ATGP4148) is coated at 2 µg/mL (100 ul/well) can bind Human EFNA5. The ED50 range ≤ 60 ng/mL.

Product Name Alternative

Ephrin-A5, EFNA5, AF1, EFL5, EPLG7, GLC1M, LERK7, RAGS, AL-1, EPH-related receptor tyrosine kinase ligand 7

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Mouse EphA3 (CAT# ATGP4148) . The ED50 range ≤ 60 ng/mL.

Molecular Weight

48.1 kDa (422aa)

Additionnal Information

ATGP4147-10 µg, ATGP4147-20 µg, ATGP4147-50 µg, ATGP4147-100 µg, ATGP4147-250 µg, ATGP4147-500 µg, ATGP4147-1 mg, ATGP4147-010, ATGP4147-020, ATGP4147-050, ATGP4147-100, ATGP4147-250, ATGP4147-500, ATGP4147-01M, ATGP4072-10 µg, ATGP4072-20 µg, ATGP4072-50 µg, ATGP4072-100 µg, ATGP4072-250 µg, ATGP4072-500 µg, ATGP4072-1 mg, ATGP4072-010, ATGP4072-020, ATGP4072-050, ATGP4072-100, ATGP4072-250, ATGP4072-500, ATGP4072-01M

References & Citations

Son AI., et al. (2013) Mol. Vis. 19:254-266.; ; Wang TH., et al. (2012) FEBS J. 279:251-263.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_001953.1

Uniprot Accession Number

P52803

Species

Human

N Terminal Sequence

Gln 21

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