Recombinant human semaphorin-4D protein

Semaphorin4D, also known as CD100, is a member of the Class 4 family of transmembrane immune and nervous system semaphorins. It is an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. It serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Sema4D, produced by T cells, activated B cells and dendritic cells acts through a low affinity receptor termed CD72 in the immune system. Unligated CD72 inhibits antigen presenting cells that express it, and this inhibition is relieved by Sema4D binding. It is reflected in its ability to inhibit ovarian carcinoma cell survival.Recombinant human semaphorin-4D, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.