Recombinant human semaphorin-4D protein

Semaphorin4D, also known as CD100, is a member of the Class 4 family of transmembrane immune and nervous system semaphorins. It is an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. It serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Sema4D, produced by T cells, activated B cells and dendritic cells acts through a low affinity receptor termed CD72 in the immune system. Unligated CD72 inhibits antigen presenting cells that express it, and this inhibition is relieved by Sema4D binding. It is reflected in its ability to inhibit ovarian carcinoma cell survival.Recombinant human semaphorin-4D, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

SEMA4D, SEMA-4D, semaphorin-4D isoform 1, semaphorin4D, C9orf164, CD100, coll-4, COLL4, M-sema-G, SEMAJ, previously Sem J, G or C-like 2, A8, BB18, GR3

Expression System

HEK293

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

106.1kDa (952aa)

Additionnal Information

ATGP4145-10 µg, ATGP4145-20 µg, ATGP4145-50 µg, ATGP4145-100 µg, ATGP4145-250 µg, ATGP4145-500 µg, ATGP4145-1 mg, ATGP4145-010, ATGP4145-020, ATGP4145-050, ATGP4145-100, ATGP4145-250, ATGP4145-500, ATGP4145-01M

References & Citations

Kumanogoh, A. and H. Kikutani (2004) Cell. Mol. Life Sci. 61:292-300.; ; Janssen, B.J.C. et al. (2010) Nature 467:1118-1122.; ; Kumanogoh, A. et al. (2005) Int. Immunol. 17:1277-1282.; ; Ishida, I. et al. (2003) Int. Immunol. 15:1027-1034.;