Recombinant human BAFF/TNFSF13B protein
B lymphocyte stimulator (BLyS), a member of the tumor necrosis factor (TNF) superfamily of cytokines, induces B-cell proliferation and immunoglobulin secretion and is a key regulator of peripheral B-cell populations in vivo.. BLyS is made by immune-cells called monocytes and macrophages. When monocytes and macrophages are activated, BLyS is released and binds to a receptor found only on B cells. Recombinant human BLyS, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human BAFF is coated at 5 µg/mL (100 ul/well) can bind Human BAFFR (CAT# ATGP4131) in a Functional ELISA assay.
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human BAFFR (CAT# ATGP4131) .
Molecular Weight
21.4 kDa (190aa)
Additionnal Information
References & Citations
Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629.; ; Michaud DS., et al. (2006), Cancer Res. 66 (8) :4525-30;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4), 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_006564.1
Uniprot Accession Number
Q9Y275
Species
Human
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
CKMT1B (Creatine Kinase, Mitochondrial 1B, CKMT, CKMT1, UMTCK) (MaxLight 750)
CKMT1B (Creatine Kinase, Mitochondrial 1B, CKMT, CKMT1, UMTCK) (MaxLight 750)
GAGs (Glycosaminoglycan) BioAssayTM ELISA Kit (Human) discontinued