Recombinant human BAFF/TNFSF13B protein

B lymphocyte stimulator (BLyS), a member of the tumor necrosis factor (TNF) superfamily of cytokines, induces B-cell proliferation and immunoglobulin secretion and is a key regulator of peripheral B-cell populations in vivo.. BLyS is made by immune-cells called monocytes and macrophages. When monocytes and macrophages are activated, BLyS is released and binds to a receptor found only on B cells. Recombinant human BLyS, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human BAFF is coated at 5 µg/mL (100 ul/well) can bind Human BAFFR (CAT# ATGP4131) in a Functional ELISA assay.

Product Name Alternative

Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, BLyS, B-cell-activating factor BAFF, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, TALL-1, CD257, TNFSF20, ZTNF4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human BAFFR (CAT# ATGP4131) .

Molecular Weight

21.4 kDa (190aa)

Additionnal Information

BAFF, BLyS, TNFSF13B, B lymphocyte stimulator, B-cell activating factor, BAFF, CD257, TALL1, THANK, ZTNF4, TNFSF13B, TNFSF20, BlyS, B lymphocyte stimulator, B lymphocyte stimulator, ATGP4139-10 µg, ATGP4139-20 µg, ATGP4139-50 µg, ATGP4139-100 µg, ATGP4139-250 µg, ATGP4139-500 µg, ATGP4139-1 mg, ATGP4139-10, ATGP4139-20, ATGP4139-50, ATGP4139-100, ATGP4139-250, ATGP4139-500, ATGP4139-1

References & Citations

Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629.; ; Michaud DS., et al. (2006), Cancer Res. 66 (8) :4525-30;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4), 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_006564.1

Uniprot Accession Number

Q9Y275

Species

Human

AA Sequence

MAV QGPEETVTQD CLQLIADSET PTIQKGSYTF VPWLLSFKRG SALEEKENKI LVKETGYFFI YGQVLYTDKT YAMGHLIQRK KVHVFGDELS LVTLFRCIQN MPETLPNNSC YSAGIAKLEE GDELQLAIPR ENAQISLDGD VTFFGALKLL