Recombinant human BAFF/TNFSF13B protein
B lymphocyte stimulator (BLyS), a member of the tumor necrosis factor (TNF) superfamily of cytokines, induces B-cell proliferation and immunoglobulin secretion and is a key regulator of peripheral B-cell populations in vivo.. BLyS is made by immune-cells called monocytes and macrophages. When monocytes and macrophages are activated, BLyS is released and binds to a receptor found only on B cells. Recombinant human BLyS, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human BAFF is coated at 5 µg/mL (100 ul/well) can bind Human BAFFR (CAT# ATGP4131) in a Functional ELISA assay.
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human BAFFR (CAT# ATGP4131) .
Molecular Weight
21.4 kDa (190aa)
Additionnal Information
References & Citations
Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629.; ; Michaud DS., et al. (2006), Cancer Res. 66 (8) :4525-30;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4), 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_006564.1
Uniprot Accession Number
Q9Y275
Species
Human
AA Sequence
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