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Recombinant human HLF protein

HLF, also known as hepatic leukemia factor, belongs to the bZIP family and the PAR (proline and acidic-rich) subfamily of transcription regulatory proteins. HLF binds DNA specifically as a homodimer or heterodimer with other PAR factors. It is highly expressed in liver, with lower levels in lung and kidney and functions as a potent trans-activator. Recombinant human HLF protein, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Hepatic leukemia factor, PAR bZIP transcription factor, E2A-HLF fusion transcript protein

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

34kDa (301aa)

Additionnal Information

ATGP4135-10 µg, ATGP4135-20 µg, ATGP4135-50 µg, ATGP4135-100 µg, ATGP4135-250 µg, ATGP4135-500 µg, ATGP4135-1 mg, ATGP4135-010, ATGP4135-020, ATGP4135-050, ATGP4135-100, ATGP4135-250, ATGP4135-500, ATGP4135-01M, ATGP1169-10 µg, ATGP1169-20 µg, ATGP1169-50 µg, ATGP1169-100 µg, ATGP1169-250 µg, ATGP1169-500 µg, ATGP1169-1 mg, ATGP1169-010, ATGP1169-020, ATGP1169-050, ATGP1169-100, ATGP1169-250, ATGP1169-500, ATGP1169-01M

References & Citations

Hunger S.P. et al. (1992) Genes Dev. 6: 1608-1620.; ; Falvey E. et al. (1995) EMBO J. 14: 4307-4317.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_002117.1

Uniprot Accession Number

Q16534

Species

Human

N Terminal Sequence

Met1

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