Recombinant human BAFF R/TNFRSF13C protein

BAFFR, also known as TNFRSF13C, is a member of the tumor necrosis factor receptor superfamily. Members of the TNF receptor superfamily (TNFRSF) have crucial roles in both innate and adaptive immunity and in cellular apoptosis process. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL, BAFFR selectively binds BAFF. The BAFFR extracellular domain lacks the CRD (TNF receptor canonical cysteine-rich domain and contains only a partial CRD with four cysteine residues. It is highly expressed in spleen, lymph node and resting B cells and is thought the principal receptor required for BAFF-mediated mature B-cell survival. It has been proposed that abnormally high levels of BAFFR may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells. Recombinant human BAFFR, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human BAFF (CAT# ATGP4139) is coated at 5 µg/mL (100 ul/well) can bind Human BAFFR. The ED50 range ≤ 0.7 µg/mL.

Product Name Alternative

Tumor necrosis factor receptor superfamily member 13C, B-cell-activating factor receptor, BAFF receptor, BAFF-R, BLyS receptor 3, BROMIX, CD268, CVID4, prolixin, TNFRSF13C, Br3

Expression System

HEK293

Antigen Species

Human

Tag

HIgG-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human BAFF (CAT# ATGP4139) . The ED50 range ≤ 0.7 µg/mL.

Molecular Weight

34.4kDa (314aa)

Additionnal Information

ATGP4131-10 µg, ATGP4131-20 µg, ATGP4131-50 µg, ATGP4131-100 µg, ATGP4131-250 µg, ATGP4131-500 µg, ATGP4131-1 mg, ATGP4131-010, ATGP4131-020, ATGP4131-050, ATGP4131-100, ATGP4131-250, ATGP4131-500, ATGP4131-01M