Recombinant human Trypsin 3/PRSS3 protein

PRSS3, also known as trypsin-3, is a member of the trypsin family of serine proteases. It is specialized for the degradation of trypsin inhibitors and may be involved in defensin processing, including DEFA5. This protein is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Compared to PRSS1 and 2, one intriguing feature of PRSS3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. It has been proposed to be degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins. Recombinant human Trypsin 3/PRSS3, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.