Recombinant human IBSP/Sialoprotein II protein

IBSP, also known as Sialoprotein II, is a monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins. It is synthesized by platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts and bone marrow stromal cells. It is a key mediator of the hypertrophic chondrocytes-induced angiogenesis. It was associated with the severity of histological cartilage lesions and with vascular density at the osteochondral junction. Recombinant human IBSP/Sialoprotein II, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human IBSP stimulates cell adhesion of the MCF7 human breast cancer cells. This effect is more to 40%.

Product Name Alternative

Bone sialoprotein II, bone sialoprotein 2, BSP II, BSP, BSP-II, SP-II, Cell-binding sialoprotein, Integrin-binding sialoprotein, IBSP, BNSP

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of MCF7 human breast cancer cells. When cells are added to Human IBSP coated plates 3 µg/mL. This effect is more to 40%.

Molecular Weight

34.3kDa (307aa)

Additionnal Information

ATGP4129-10 µg, ATGP4129-20 µg, ATGP4129-50 µg, ATGP4129-100 µg, ATGP4129-250 µg, ATGP4129-500 µg, ATGP4129-1 mg, ATGP4129-010, ATGP4129-020, ATGP4129-050, ATGP4129-100, ATGP4129-250, ATGP4129-500, ATGP4129-01M, ATGP3388-10 µg, ATGP3388-20 µg, ATGP3388-50 µg, ATGP3388-100 µg, ATGP3388-250 µg, ATGP3388-500 µg, ATGP3388-1 mg, ATGP3388-010, ATGP3388-020, ATGP3388-050, ATGP3388-100, ATGP3388-250, ATGP3388-500, ATGP3388-01M