Recombinant human IBSP/Sialoprotein II protein
IBSP, also known as Sialoprotein II, is a monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins. It is synthesized by platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts and bone marrow stromal cells. It is a key mediator of the hypertrophic chondrocytes-induced angiogenesis. It was associated with the severity of histological cartilage lesions and with vascular density at the osteochondral junction. Recombinant human IBSP/Sialoprotein II, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IBSP stimulates cell adhesion of the MCF7 human breast cancer cells. This effect is more to 40%.
Product Name Alternative
Bone sialoprotein II, bone sialoprotein 2, BSP II, BSP, BSP-II, SP-II, Cell-binding sialoprotein, Integrin-binding sialoprotein, IBSP, BNSP
Expression System
HEK293
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
34.3kDa (307aa)
Additionnal Information
References & Citations
Pesesse L., et al. (2014) Osteoarthritis Cartilage. 22:547-556.; ; Kruger TE., et al. (2014) Crit Rev Oncol Hematol. 89:330-341.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction, Cytoskeleton
NCBI Accession Number
NP_004958.2
Uniprot Accession Number
P21815
Species
Human
N Terminal Sequence
Phe 17
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