Recombinant human Insulysin/IDE protein
Insulysin/IDE, also known insulin-degrading enzyme, is a zinc metallopeptidase of the inverzincin family. This enzyme catalyses the degradation reaction of insulin, glucagon and other polypeptides. It is present in mammals and in many arthropods such as the fly Drosophila melanogaster. It is also expressed in many tissues, with the highest levels in liver, kidney, brain, and testis. It has been shown to degrade the amyloid beta peptide, which polymerizes into the plaques associated with Alzheimers disease. Therefore, the deficiency of IDE activity may contribute to the pathogenesis of type 2 diabetes mellitus and Alzheimers disease. Recombinant human Insulysin/IDE, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Insulin-degrading enzyme, insulin-degrading enzyme isoform 1, Abeta-degrading protease, Insulin protease, Insulysin, IDE
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
114kDa (984aa)
Additionnal Information
References & Citations
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 0.05% Brij35, 10% glycerol
Scientific Category
Enzymes & Proteases, Neuroscience
NCBI Accession Number
NP_004960.2
Uniprot Accession Number
P14735
Species
Human
N Terminal Sequence
Met 42
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