Recombinant human Insulysin/IDE protein

Insulysin/IDE, also known insulin-degrading enzyme, is a zinc metallopeptidase of the inverzincin family. This enzyme catalyses the degradation reaction of insulin, glucagon and other polypeptides. It is present in mammals and in many arthropods such as the fly Drosophila melanogaster. It is also expressed in many tissues, with the highest levels in liver, kidney, brain, and testis. It has been shown to degrade the amyloid beta peptide, which polymerizes into the plaques associated with Alzheimers disease. Therefore, the deficiency of IDE activity may contribute to the pathogenesis of type 2 diabetes mellitus and Alzheimers disease. Recombinant human Insulysin/IDE, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 3,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-RPPGFSAFK (Dnp) -OH to MCA-Pro-Leu-OH per minute at pH 7.5 at 25°C.

Product Name Alternative

Insulin-degrading enzyme, insulin-degrading enzyme isoform 1, Abeta-degrading protease, Insulin protease, Insulysin, IDE

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 3,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-RPPGFSAFK (Dnp) -OH to MCA-Pro-Leu-OH per minute at pH 7.5 at 25°C.

Molecular Weight

114kDa (984aa)

Additionnal Information

ATGP4128-10 µg, ATGP4128-20 µg, ATGP4128-50 µg, ATGP4128-100 µg, ATGP4128-250 µg, ATGP4128-500 µg, ATGP4128-1 mg, ATGP4128-010, ATGP4128-020, ATGP4128-050, ATGP4128-100, ATGP4128-250, ATGP4128-500, ATGP4128-01M

References & Citations

Affholter J. A., et al. (1988) Science. 242:1415-1418.; ; Duckworth W.C., et al. (1998) Endocr. Rev. 19:608-624.; ; Akiyama H., et al. (1990) Biochem. Biophys. Res. Commun. 170:1325-1330.