Recombinant mouse MMP-9 protein

MMP-9, also known matrix metalloproteinase-9, is one of the matrix metalloproteinases superfamily which is zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. It degrades many substrates such as gelatin, collagens, elastin and proteoglycan core protein which appears to be involved in invasive ability. This protein also plays an essential role in leukocyte migration and in bone osteoclastic resorption. It plays an important role in angiogenesis and neovascularization and so appears to be involved in the remodeling associated with malignant glioma neovascularization. Recombinant mouse MMP-9 protein, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >1,500 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 7.5 at 25C.

Product Name Alternative

Matrix metalloproteinase-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, Mmp9, MANDP2, AW743869, B/MMP, B/MMP9, Clg4, Clg4b, Gel B, MMP-9, pro-MMP-9

Expression System

HEK293

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is >1,500 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 7.5 at 25C.

Molecular Weight

79.3kDa (717aa)

Additionnal Information

ATGP4127-10 µg, ATGP4127-20 µg, ATGP4127-50 µg, ATGP4127-100 µg, ATGP4127-250 µg, ATGP4127-500 µg, ATGP4127-1 mg, ATGP4127-010, ATGP4127-020, ATGP4127-050, ATGP4127-100, ATGP4127-250, ATGP4127-500, ATGP4127-01M, ATGP4087-10 µg, ATGP4087-20 µg, ATGP4087-50 µg, ATGP4087-100 µg, ATGP4087-250 µg, ATGP4087-500 µg, ATGP4087-1 mg, ATGP4087-010, ATGP4087-020, ATGP4087-050, ATGP4087-100, ATGP4087-250, ATGP4087-500, ATGP4087-01M