Recombinant mouse MMP-9 protein
MMP-9, also known matrix metalloproteinase-9, is one of the matrix metalloproteinases superfamily which is zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. It degrades many substrates such as gelatin, collagens, elastin and proteoglycan core protein which appears to be involved in invasive ability. This protein also plays an essential role in leukocyte migration and in bone osteoclastic resorption. It plays an important role in angiogenesis and neovascularization and so appears to be involved in the remodeling associated with malignant glioma neovascularization. Recombinant mouse MMP-9 protein, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is >1,500 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 7.5 at 25C.
Product Name Alternative
Expression System
HEK293
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is >1,500 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 7.5 at 25C.
Molecular Weight
79.3kDa (717aa)
Additionnal Information
References & Citations
Lee YD., et al, (2014) BMB Rep. 47:262-267.; ; Matin S., et al, (2018) Int J Chron Obstruct Pulmon Dis. 13:1449-1454.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl (pH 7.5) containing 1mM CaCl2, 100 mM NaCl, 10% glycerol
Scientific Category
Angiogenesis, Cardiovascular, Adhesion & Movement
NCBI Accession Number
NP_038627.1
Uniprot Accession Number
P41245
Species
Mouse
N Terminal Sequence
Ala
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