What sizes are available for Recombinant human Alkaline Phosphatase/ALPL protein?

Recombinant human Alkaline Phosphatase/ALPL protein is available in the following size(s): 50 µg.

Recombinant human Alkaline Phosphatase/ALPL protein

Q: What is the catalog number for Recombinant human Alkaline Phosphatase/ALPL protein?

The catalog number for Recombinant human Alkaline Phosphatase/ALPL protein is ATGP4125-050.

Q: Is Recombinant human Alkaline Phosphatase/ALPL protein in stock?

Recombinant human Alkaline Phosphatase/ALPL protein is currently made to order / not held in immediate stock. Contact us for lead time.

ALPL, also known as alkaline phosphatase, tissue-nonspecific isozyme, is one of alkaline phosphatases. Alkaline phosphatase is metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue-nonspecific) . Expression of this protein is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. It plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate. It thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration. Recombinant human Alkaline Phosphatase/ALPL, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 40,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 37C.

Product Name Alternative

Aalkaline phosphatase, tissue-nonspecific isozyme isoform 1, Alkaline phosphatase liver/bone/kidney isozyme, Phosphoamidase, Phosphocreatine phosphatase, ALPL, AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Molecular Weight

54.3kDa (493aa)

Additionnal Information

ATGP4125-10 µg, ATGP4125-20 µg, ATGP4125-50 µg, ATGP4125-100 µg, ATGP4125-250 µg, ATGP4125-500 µg, ATGP4125-1 mg, ATGP4125-010, ATGP4125-020, ATGP4125-050, ATGP4125-100, ATGP4125-250, ATGP4125-500, ATGP4125-01M

References & Citations

Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1-39.; ; Sara Sultana., et al, (2013) Mol Genet Metab. 109:282-288.; ; Sonia Di Mauro., et al, (2002) J Bone Miner Res. 17:1383-1391.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Enzymes & Proteases, Cell Cycle

NCBI Accession Number

NP_000469.3

Uniprot Accession Number

P05186

Species

Human

N Terminal Sequence

Asp

AA Sequence

LVPEKEK DPKYWRDQAQ ETLKYALELQ KLNTNVAKNV IMFLGDGMGV STVTAARILK GQLHHNPGEE TRLEMDKFPF VALSKTYNTN AQVPDSAGTA TAYLCGVKAN EGTVGVSAAT ERSRCNTTQG NEVTSILRWA KDAGKSVGIV TTTRVNHATP SAAYAHSADR DWYSDNEMPP EALSQGCKDI AYQLMHNIRD IDVIMGGGRK YMYPKNKTDV EYESDEKARG TRLDGLDLVD TWKSFKPRYK HSHFIWNRTE LLTLDPHNVD YLLGLFEPGD MQYELNRNNV TDPSLSEMVV VAIQILRKNP KGFFLLVEGG RIDHGHHEGK AKQALHEAVE MDRAIGQAGS LTSSEDTLTV VTADHSHVFT FGGYTPRGNS IFGLAPMLSD TDKKPFTAIL YGNGPGYKVV GGERENVSMV DYAHNNYQAQ SAVPLRHETH GGEDVAVFSK GPMAHLLHGV HEQNYVPHVM AYAACIGANL GHCAPAS