Recombinant Human Fc epsilon RI alpha protein

Fc epsilon RI alpha, also known as FCER1A, is an IgE‑binding protein of the multichain immune recognition family. Fc epsilon receptor is a tetrameric complex of one alpha, one beta and two gamma subunits on mast cells and basophils. This protein represents the alpha subunit, only the alpha subunit is glycosylated. The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes—potent effectors of the hypersensitivity response. Recombinant human Fc epsilon RI alpha/FCER1A, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.