Recombinant human DSPG3 protein

DSPG3, also called epiphycan/EPYC or PG-Lb, is a class III subfamily member of the SLRP (small leucine-rich proteoglycans) family. DSPG3 is found predominantly in epiphyseal cartilage, although DSPG3 mRNA is also detected in mouse testis and human ligament and placenta. In mouse, expression begins in mid-gestation as an intermediate marker for chondrogenesis, peaks at birth and declines thereafter. Deletion of DSPG3 in mouse results in a mild postnatal phenotype that worsens synergistically when the co‑expressed SLRP biglycan is also deleted. The double-mutant phenotype includes premature osteoarthritis, indicating a role for DSPG3, in cooperation with other SLRPs, in stability of the collagen matrix and maintenance of joint integrity. Recombinant human DSPG3, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human DSPG3 and human fibronectin stimulates cell adhesion of the Saos‑2 human osteosarcoma cells. This effect is more to 60%.

Product Name Alternative

Epiphycan, Dermatan sulfate proteoglycan 3, Proteoglycan-Lb, PG-Lb, Small chondroitin/dermatan sulfate proteoglycan, EPYC, DSPG3, PGLB, SLRR3B, epiphycan proteoglycan

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of Saos‑2 human osteosarcoma cells. When cells are added to human DSPG3 1.25 µg/mL and human fibronectin 0.5 µg/mL coated plates. This effect is more to 60%.

Molecular Weight

35.5kDa (309aa)

Additionnal Information

ATGP4123-10 µg, ATGP4123-20 µg, ATGP4123-50 µg, ATGP4123-100 µg, ATGP4123-250 µg, ATGP4123-500 µg, ATGP4123-1 mg, ATGP4123-010, ATGP4123-020, ATGP4123-050, ATGP4123-100, ATGP4123-250, ATGP4123-500, ATGP4123-01M

References & Citations

Michelle Deere., et al, (1999) Genome Res. 9:449-456.; ; Jan Johnson., et al, (1999) Dev. Dyn. 216:499-510.; ; S. Nuka., et al, (2010) Osteoarthritis Cartilage 18:88-96.