Recombinant human APP/Protease Nexin II protein

APP, also known as amyloid beta A4 protein, functions as a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. It has been implicated as a regulator of synapse formation, neural plasticity, antimicrobial activity, and iron export. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebral arterial amyloidosis. Recombinant human APP, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

The IC50 value is ≤ 1 nM, The inhibitory function of APP on activity of trypsin was measured by a fluorometric assay using Mca-RPKPVE-Nval-WRK (Dnp) -NH2 at pH 7.5 at 37C.

Product Name Alternative

ABPP, APPI, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta precursor protein, Amyloid-beta A4 protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, A4, AD1, beta-amyloid peptide, beta-amyloid precursor protein, testicular tissue protein Li 2, AAA, ABETA, alpha-sApp, CTF gamma, PN2

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

The IC50 value is ≤ 1 nM, The inhibitory function of APP on activity of trypsin was measured by a fluorometric assay using Mca-RPKPVE-Nval-WRK (Dnp) -NH2 at pH 7.5 at 37C.

Molecular Weight

78.2kDa (690aa)

Additionnal Information

ATGP4122-10 µg, ATGP4122-20 µg, ATGP4122-50 µg, ATGP4122-100 µg, ATGP4122-250 µg, ATGP4122-500 µg, ATGP4122-1 mg, ATGP4122-010, ATGP4122-020, ATGP4122-050, ATGP4122-100, ATGP4122-250, ATGP4122-500, ATGP4122-01M