Recombinant human HAPLN1 protein

HAPLN1, also known as CRTL1, is a member of the hyaladherin family. This protein contains a common structural domain of about 100 amino acids that is termed a Link module with two α-helices and two antiparallel β-sheets. It involved in the formation and stability of extracellular matrix via its association with specific HA-binding proteins. It stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix. Also, It has been shown to interact with Versican, two extracellular matrix components essential for cardiac development. Recombinant human HAPLN1, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Hyaluronic acid is coated at 10 µg/mL (100 ul/well) can bind Human HAPLN1. The ED50 range ≤ 1 µg/mL.

Product Name Alternative

Hyaluronan and proteoglycan link protein 1, CRT1, CRTL1, HPLN1, Cartilage-linking protein 1, Proteoglycan link protein

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Hyaluronic acid. The ED50 range ≤ 1 µg/mL.

Molecular Weight

39.3kDa (345aa)

Additionnal Information

ATGP4121-10 µg, ATGP4121-20 µg, ATGP4121-50 µg, ATGP4121-100 µg, ATGP4121-250 µg, ATGP4121-500 µg, ATGP4121-1 mg, ATGP4121-010, ATGP4121-020, ATGP4121-050, ATGP4121-100, ATGP4121-250, ATGP4121-500, ATGP4121-01M

References & Citations

Zhang T., et al. (2022) Gastric Cancer. 25:346-359.; ; Sun J., et al. (2022) Circulation. 146:48-63.; ; Day, A.J. and G.D. Prestwich (2002) J. Biol. Chem. 277:4585-4588.