Recombinant human HAPLN1 protein
HAPLN1, also known as CRTL1, is a member of the hyaladherin family. This protein contains a common structural domain of about 100 amino acids that is termed a Link module with two α-helices and two antiparallel β-sheets. It involved in the formation and stability of extracellular matrix via its association with specific HA-binding proteins. It stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix. Also, It has been shown to interact with Versican, two extracellular matrix components essential for cardiac development. Recombinant human HAPLN1, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Hyaluronic acid is coated at 10 µg/mL (100 ul/well) can bind Human HAPLN1. The ED50 range ≤ 1 µg/mL.
Product Name Alternative
Hyaluronan and proteoglycan link protein 1, CRT1, CRTL1, HPLN1, Cartilage-linking protein 1, Proteoglycan link protein
Expression System
HEK293
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Hyaluronic acid. The ED50 range ≤ 1 µg/mL.
Molecular Weight
39.3kDa (345aa)
Additionnal Information
References & Citations
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Scientific Category
Signal Transduction, Cytoskeleton
NCBI Accession Number
NP_001875.1
Uniprot Accession Number
P10915
Species
Human
N Terminal Sequence
Asp
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