Recombinant mouse IL-17A protein

IL-17A, also known as CTLA‑8, is a cytokine that belongs to the Interleukin‑17 family. It produced by activated T cells and plays an important role in anti‑microbial and chronic inflammation. Mouse IL‑17A shares 61% amino acid sequence identity with human IL‑17A. It promotes protective mucosal and epidermal inflammation in response to microbial infection and induces chemokine production, neutrophil influx, and the production of antibacterial peptides. It can stimulate the expression of IL-6 and PTGS2/COX-2, as well as enhance the production of nitric oxide (NO) . High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis. Also, IL‑17A has been shown to exert either tumorigenic or anti‑tumor effects. Recombinant mouse IL-17A, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse IL-17A stimulates IL-6 secretion of the NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 2 ng/mL.

Product Name Alternative

Interleukin-17A, IL17A, IL-17, IL-17A, Il17, Cytotoxic T-lymphocyte-associated antigen 8. Ctla-8, Ctla8

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

The activity is determined by the IL-6 ELISA in a using NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 2 ng/mL.

Molecular Weight

16kDa (141aa)

Additionnal Information

ATGP4120-10 µg, ATGP4120-20 µg, ATGP4120-50 µg, ATGP4120-100 µg, ATGP4120-250 µg, ATGP4120-500 µg, ATGP4120-1 mg, ATGP4120-010, ATGP4120-020, ATGP4120-050, ATGP4120-100, ATGP4120-250, ATGP4120-500, ATGP4120-01M, ATGP3280-10 µg, ATGP3280-20 µg, ATGP3280-50 µg, ATGP3280-100 µg, ATGP3280-250 µg, ATGP3280-500 µg, ATGP3280-1 mg, ATGP3280-010, ATGP3280-020, ATGP3280-050, ATGP3280-100, ATGP3280-250, ATGP3280-500, ATGP3280-01M