Recombinant mouse ICAM-1/CD54 protein
ICAM-1, also known as CD54 (Cluster of Differentiation 54), belongs to the ICAM proteins. This protein is ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) . When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. During leukocyte trans-endothelial migration, ICAM-1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. It is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. Also, It has been implicated in the progression of autoimmune diseases. Recombinant mouse ICAM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse ICAM-1/CD54 stimulates cell adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cells. This effect is more to 60%.
Product Name Alternative
ICAM1, CD54, Icam-1, Ly-47, MALA-2, Intercellular adhesion molecule 1, MyD10
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
51.2 kDa (466aa)
Additionnal Information
References & Citations
Siu G., et al. (1989) J. Immunol. 143:3813-3820.; ; Lord K. A., et al. (1990) Oncogene 5:387-396.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology, Adhesion & Movement
NCBI Accession Number
NP_034623.1
Uniprot Accession Number
P13597
Species
Mouse
N Terminal Sequence
Gln 28
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