Recombinant mouse ICAM-1/CD54 protein

ICAM-1, also known as CD54 (Cluster of Differentiation 54), belongs to the ICAM proteins. This protein is ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) . When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. During leukocyte trans-endothelial migration, ICAM-1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. It is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. Also, It has been implicated in the progression of autoimmune diseases. Recombinant mouse ICAM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse ICAM-1/CD54 stimulates cell adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cells. This effect is more to 60%.

Product Name Alternative

ICAM1, CD54, Icam-1, Ly-47, MALA-2, Intercellular adhesion molecule 1, MyD10

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM-1/CD54 coated plates 5 µg/mL. This effect is more to 60%.

Molecular Weight

51.2 kDa (466aa)

Additionnal Information

ATGP4119-10 µg, ATGP4119-20 µg, ATGP4119-50 µg, ATGP4119-100 µg, ATGP4119-250 µg, ATGP4119-500 µg, ATGP4119-1 mg, ATGP4119-010, ATGP4119-020, ATGP4119-050, ATGP4119-100, ATGP4119-250, ATGP4119-500, ATGP4119-01M, ATGP3268-10 µg, ATGP3268-20 µg, ATGP3268-50 µg, ATGP3268-100 µg, ATGP3268-250 µg, ATGP3268-500 µg, ATGP3268-1 mg, ATGP3268-010, ATGP3268-020, ATGP3268-050, ATGP3268-100, ATGP3268-250, ATGP3268-500, ATGP3268-01M