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Recombinant mouse Lactate Dehydrogenase A/LDHA protein

Ldha, also known as L-lactate dehydrogenase A chain isoform 1, is a member of the LDH/MDH superfamily and LDH family. It catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. This protein is found predominantly in muscle tissue. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It plays an important role in the development, invasion and metastasis of malignancies. Recombinant mouse Ldha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 250unit/mg, and is defined as the Amount of enzyme that convert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37°C.

Product Name Alternative

L-lactate dehydrogenase A chain isoform 1, LDH muscle subunit (LDH-M), Ldha, 17R2, Ldh1, Ldhm, LDH-A, LDH muscle smb

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 250unit/mg, and is defined as the Amount of enzyme that convert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37°C.

Molecular Weight

37.5 kDa (340aa)

Additionnal Information

ATGP4118-10 µg, ATGP4118-20 µg, ATGP4118-50 µg, ATGP4118-100 µg, ATGP4118-250 µg, ATGP4118-500 µg, ATGP4118-1 mg, ATGP4118-010, ATGP4118-020, ATGP4118-050, ATGP4118-100, ATGP4118-250, ATGP4118-500, ATGP4118-01M, ATGP3341-10 µg, ATGP3341-20 µg, ATGP3341-50 µg, ATGP3341-100 µg, ATGP3341-250 µg, ATGP3341-500 µg, ATGP3341-1 mg, ATGP3341-010, ATGP3341-020, ATGP3341-050, ATGP3341-100, ATGP3341-250, ATGP3341-500, ATGP3341-01M

References & Citations

Rong Y., et al. (2013) Tumour Biol. 34 (3) :1523-1530.; ; Merkle S., et al. (1992) Genetics. 131 (2) :413-421.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction, Metabolism

NCBI Accession Number

NP_034829

Uniprot Accession Number

P06151

Species

Mouse

N Terminal Sequence

Met1

Frequently Asked Questions

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