Recombinant human FGF basic/FGF-2 protein

Fibroblast growth factor 2 (FGF2) is a member of the FGF family that binds heparin and plays important roles in morphogenic, neurotrophic and angiogenic processes. FGF2 possess diverse biological functions, such as neuron differentiation, embryonic development and differentiation, modulation of angiogenesis, and wound healing. Recombinant human FGF2 was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Human FGF-2 stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 µg/mL of heparin. The ED50 range ≤ to 0.2 ng/mL.

Product Name Alternative

Fibroblast growth factor 2, BFGF, FGFB, HBGF-2, FGF2, FGF-2

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 µg/mL of heparin. The ED50 range ≤ 0.2 ng/mL.

Molecular Weight

16.5 kDa (147aa) confirmed by MALDI-TOF

Additionnal Information

FGF basic, Fibroblast growth factor 2, BFGF, FGFB, HBGF-2, ATGP4117-10 µg, ATGP4117-20 µg, ATGP4117-50 µg, ATGP4117-100 µg, ATGP4117-250 µg, ATGP4117-500 µg, ATGP4117-1 mg, ATGP4117-10, ATGP4117-20, ATGP4117-50, ATGP4117-100, ATGP4117-250, ATGP4117-500, ATGP4117-1

References & Citations

Nakayama T., et al. (2007) Blood. 109 (2) :1363-72. ; ; A E Eriksson., et al. (1991) PNAS. 88:3441-3445; ; Raines, E.W. et al. (1985) Methods Enzymol. 109:749-73

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 50 mM Tris-HCl buffer (pH 8.0)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_001997.5

Uniprot Accession Number

P09038

Species

Human

AA Sequence

MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS