Recombinant human Carbonic Anhydrase X/CA10 protein

Carbonic anhydrase X, also known as CA10, belongs to the CA family of zinc metalloenzymes. It is catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. Also an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development. Recombinant human Carbonic Anhydrase X/CA10, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 150 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH8.0 at 37°C.

Product Name Alternative

Carbonic anhydrase-related protein 10, Carbonic anhydrase-related protein X, CA-RP X, CARP X, carbonic anhydrase X, Cerebral protein 15, hucep-15, UNQ533/PRO1076, epididymis secretory sperm binding protein

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 150 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH8.0 at 37°C.

Molecular Weight

36.3kDa (317aa)

Additionnal Information

ATGP4115-10 µg, ATGP4115-20 µg, ATGP4115-50 µg, ATGP4115-100 µg, ATGP4115-250 µg, ATGP4115-500 µg, ATGP4115-1 mg, ATGP4115-010, ATGP4115-020, ATGP4115-050, ATGP4115-100, ATGP4115-250, ATGP4115-500, ATGP4115-01M

References & Citations

Taniuchi K., et al, (2002) Brain Res. Mol. Brain Res. 109:207.; ; Okamoto N., et al, (2001) Biochim. Biophys. Acta. 1518:311-316.; ; Hewett-Emmett, D. and R.E Tashian (1996) Mol. Phylogenet. Evol. 5:50-77.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_064563.1

Uniprot Accession Number

Q9NS85

Species

Human

N Terminal Sequence

Asp

AA Sequence

QQNSPK IHEGWWAYKE VVQGSFVPVP SFWGLVNSAW NLCSVGKRQS PVNIETSHMI FDPFLTPLRI NTGGRKVSGT MYNTGRHVSL RLDKEHLVNI SGGPMTYSHR LEEIRLHFGS EDSQGSEHLL NGQAFSGEVQ LIHYNHELYT NVTEAAKSPN GLVVVSIFIK VSDSSNPFLN RMLNRDTITR ITYKNDAYLL QGLNIEELYP ETSSFITYDG SMTIPPCYET ASWIIMNKPV YITRMQMHSL RLLSQNQPSQ IFLSMSDNFR PVQPLNNRCI RTNINFSLQG KDCPNNRAQK LQYRVNEWLL K