Recombinant mouse BACE-2 protein
BACE-2, also known as beta secretase 2, belongs to the peptidase A1 family. It is an aspartic protease that is a close homolog of BACE-1. However, BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in Aβ peptide production. It has been shown to play a key role in insulin receptor trafficking in the pancreas where it is expressed in β cells and affects glucose tolerance and was suggested as a promising target for improving β cell function in diabetes. Recombinant mouse BACE-2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Expression System
HEK293
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
48.6kDa (449aa)
Additionnal Information
References & Citations
Sun, X. et al. (2006) FASEB J. 19:739-749.; ; Hussain, I. et al. (2001) J. Biol. Chem. 276:23322-23328.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Protease inhibitors, Neuroscience
NCBI Accession Number
NP_062390.3
Uniprot Accession Number
Q9JL18
Species
Mouse
N Terminal Sequence
Ala 20
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