Recombinant human CD26/DPP4 protein

DPP4, dipeptidyl peptidase-4 is a complex enzyme expressed on the surface of most cell types and is a serine exopeptidase that cleaves x-proline dipeptides from the N-terminus of polypeptides. DPP4 protein is associated with intracellular signal transduction, apoptosis and plays an important role in tumor biology. There are at least 63 substrates which can bind specifically to DPP4 enzyme including growth factors, chemokines, neuro peptides. Furthermore, DPP4 plays a major role in glucose metabolism by cleaving incretins such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) . Middle East respiratory syndrome coronavirus has been found to bind to DPP4. It is found on the surface of cells in the airways and kidneys. Recombinant human DPPIV/CD26, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

MERS-CoV Spike S1 Subunit (CAT# ATGP3980) is coated at 2 µg/mL (100 ul/well) can bind IgK-DPP4. The ED50 range ≤250 ng/mL.MERS-CoV Spike RBD (CAT# ATGP4111) is coated at 2 µg/mL (100 ul/well) can bind IgK-DPP4. The ED50 range ≤200 ng/mL.{2301300002142024.png}MERS-CoV Spike (CAT# ATGP4112) is coated at 2 µg/mL (100 ul/well) can bind IgK-DPP4. The ED50 range ≤120 ng/mL.{2301300002462856.png}

Product Name Alternative

Dipeptidyl peptidase 4, dipeptidyl peptidase 4 isoform 1, ADABP, ADCP2, CD26, DPPIV, TP103, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase IV, DPP4, T-cell activation antigen CD26

Expression System

HEK293

Antigen Species

Human

Tag

HIgG-Tag

Applications

SDS-PAGE, Enzyme Activity, Bioactivity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

1.Measured by its binding ability in a functional ELISA with MERS-CoV Spike S1 Subunit (CAT# ATGP3980) . The ED50 range ≤250 ng/mL.; ;2.Measured by its binding ability in a functional ELISA with MERS-CoV Spike RBD (CAT# ATGP4111) . The ED50 range ≤200 ng/mL.; ;3.Measured by its binding ability in a functional ELISA with MERS-CoV Spike (CAT# ATGP4112) . The ED50 range ≤120 ng/mL.; ;4.Specific activity is > 15,000 pmol/min/ug in which one unit defined as the amount of enzyme that hydrolyze 1pmole of H-Gly-Pro-AMC.HBr to H-Gly-Pro and AMC per minute at pH 8.0 at 37°C.

Molecular Weight

112.1kDa (977aa)

Additionnal Information

ATGP4109-10 µg, ATGP4109-20 µg, ATGP4109-50 µg, ATGP4109-100 µg, ATGP4109-250 µg, ATGP4109-500 µg, ATGP4109-1 mg, ATGP4109-10, ATGP4109-20, ATGP4109-50, ATGP4109-100, ATGP4109-250, ATGP4109-500, ATGP4109-1

References & Citations

Pratley RE and Salsali A. (2007) Curr Med Res Opin. 23 (4) :919-31.; ; Rosenstock J. and Zinman B. (2007) Curr Opin Endocrino Diabetes Obes.60 (11) :1454-70.; ; Barnett A. (2006) . J.Clin. Pract. 60 (11) :1454-70.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Angiogenesis, Cardiovascular, Adhesion & Movement

NCBI Accession Number

NP_001926.2

Uniprot Accession Number

P27487

Species

Human

N Terminal Sequence

Asp

AA Sequence

NKGTDD ATADSRKTYT LTDYLKNTYR LKLYSLRWIS DHEYLYKQEN NILVFNAEYG NSSVFLENST FDEFGHSIND YSISPDGQFI LLEYNYVKQW RHSYTASYDI YDLNKRQLIT EERIPNNTQW VTWSPVGHKL AYVWNNDIYV KIEPNLPSYR ITWTGKEDII YNGITDWVYE EEVFSAYSAL WWSPNGTFLA YAQFNDTEVP LIEYSFYSDE SLQYPKTVRV PYPKAGAVNP TVKFFVVNTD SLSSVTNATS IQITAPASML IGDHYLCDVT WATQERISLQ WLRRIQNYSV MDICDYDESS GRWNCLVARQ HIEMSTTGWV GRFRPSEPHF TLDGNSFYKI ISNEEGYRHI CYFQIDKKDC TFITKGTWEV IGIEALTSDY LYYISNEYKG MPGGRNLYKI QLSDYTKVTC LSCELNPERC QYYSVSFSKE AKYYQLRCSG PGLPLYTLHS SVNDKGLRVL EDNSALDKML QNVQMPSKKL DFIILNETKF WYQMILPPHF DKSKKYPLLL DVYAGPCSQK ADTVFRLNWA TYLASTENII VASFDGRGSG YQGDKIMHAI NRRLGTFEVE DQIEAARQFS KMGFVDNKRI AIWGWSYGGY VTSMVLGSGS GVFKCGIAVA PVSRWEYYDS VYTERYMGLP TPEDNLDHYR NSTVMSRAEN FKQVEYLLIH GTADDNVHFQ QSAQISKALV DVGVDFQAMW YTDEDHGIAS STAHQHIYTH MSHFIKQCFS LP