Recombinant human CD4 protein

CD4, also known as T-cell surface antigen T4/Leu-3, is a member of the immunoglobulin superfamily. It is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. It is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Also, it binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. Recombinant human CD4, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CD4 stimulates cell adhesion of the HeLa human cervical epithelial carcinoma cells. The ED50 range ≤ 10 µg/mL.

Product Name Alternative

T-cell surface glycoprotein CD4, CD4, CD4mut, T-cell surface glycoprotein CD4 isoform 1, T-cell surface antigen T4/Leu-3, OKT4D

Expression System

HEK293

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

<1 EU per 1ug of protein (determined by LAL method)

Purity

90% by SDS - PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. When cells are added to human CD4 coated plates. The ED50 range ≤ 10 µg/mL.

Molecular Weight

67.7kDa (604aa)

Additionnal Information

ATGP4108-10 µg, ATGP4108-20 µg, ATGP4108-50 µg, ATGP4108-100 µg, ATGP4108-250 µg, ATGP4108-500 µg, ATGP4108-1 mg, ATGP4108-10, ATGP4108-20, ATGP4108-50, ATGP4108-100, ATGP4108-250, ATGP4108-500, ATGP4108-1

References & Citations

Singh SK., et al. (2012) FEBS J. 279:3705-3714.; ; Farrar WL., et al. (1988) Crit Rev Immunol. 8:315-339.; ; Doyle, C. and J.L. Strominger (1987) Nature 330:256-259.;