Recombinant human POR protein
POR, also known as NADPH--cytochrome P450 reductase, is a flavoprotein that donates electrons to all microsomal P450 enzymes. POR is localized to the endoplasmic reticulum, where it is also able to transfer electrons to heme oxygenase and cytochrome b5. It is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase and flavodoxin. Recombinant human POR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
NADPH--cytochrome P450 reductase, NADPH--cytochrome P450 reductase, CPR, CYPOR, P450R
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
73kDa (642aa)
Additionnal Information
References & Citations
Shen A L, et al. (1989) . J Biol Chem. 264:7584-7589.; ; Haniju M, et al. (1989) . Biochemistry. 28:8639-8645.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cardiovascular
NCBI Accession Number
NP_001382342.1
Uniprot Accession Number
P16435
Species
Human
N Terminal Sequence
Met
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