Recombinant human POR protein

POR, also known as NADPH--cytochrome P450 reductase, is a flavoprotein that donates electrons to all microsomal P450 enzymes. POR is localized to the endoplasmic reticulum, where it is also able to transfer electrons to heme oxygenase and cytochrome b5. It is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase and flavodoxin. Recombinant human POR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 1,500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole cytochrome C by NADPH per minute at pH8.0 at 25C.

Product Name Alternative

NADPH--cytochrome P450 reductase, NADPH--cytochrome P450 reductase, CPR, CYPOR, P450R

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 1,500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole cytochrome C by NADPH per minute at pH8.0 at 25C.

Molecular Weight

73kDa (642aa)

Additionnal Information

ATGP4107-10 µg, ATGP4107-20 µg, ATGP4107-50 µg, ATGP4107-100 µg, ATGP4107-250 µg, ATGP4107-500 µg, ATGP4107-1 mg, ATGP4107-10, ATGP4107-20, ATGP4107-50, ATGP4107-100, ATGP4107-250, ATGP4107-500, ATGP4107-1