Recombinant human IGFBP-1 protein
IGFBP-1 (Insulin-like growth factor-binding protein 1), also known as PP12 (placental protein 12), is a member of the Insulin-like growth factor-binding protein family. This protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-1 is expressed in liver, decidua, kidneys and is the most abundant IGFBP in amniotic fluid. Serum levels of IGFBP-1 are lowest after meals. Hepatocyte production of IGFBP-1 is regulated at the transcriptional level due to the affects of insulin and corticosteriods. Recombinant human IGFBP-1 was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IGFBP-1 inhibit proliferation using MCF-7 human breast cancer cells in the presence of Human IGF-1. The ED50 range ≤3 µg/mL.
Product Name Alternative
Expression System
HEK293
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its ability to inhibit proliferation using MCF-7 human breast cancer cells in the presence of Human IGF-1. The ED50 range ≤3 µg/mL.
Molecular Weight
25.2kDa (234aa)
Additionnal Information
References & Citations
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000587.1
Uniprot Accession Number
P08833
Species
Human
N Terminal Sequence
Ala26
AA Sequence
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