What is the catalog number for Recombinant human IGFBP-1 protein?

The catalog number for Recombinant human IGFBP-1 protein is ATGP4103-050.

What sizes are available for Recombinant human IGFBP-1 protein?

Recombinant human IGFBP-1 protein is available in the following size(s): 50 µg.

Is Recombinant human IGFBP-1 protein in stock?

Recombinant human IGFBP-1 protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human IGFBP-1 protein

IGFBP-1 (Insulin-like growth factor-binding protein 1), also known as PP12 (placental protein 12), is a member of the Insulin-like growth factor-binding protein family. This protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-1 is expressed in liver, decidua, kidneys and is the most abundant IGFBP in amniotic fluid. Serum levels of IGFBP-1 are lowest after meals. Hepatocyte production of IGFBP-1 is regulated at the transcriptional level due to the affects of insulin and corticosteriods. Recombinant human IGFBP-1 was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human IGFBP-1 inhibit proliferation using MCF-7 human breast cancer cells in the presence of Human IGF-1. The ED50 range ≤3 µg/mL.

Product Name Alternative

Insulin like growth factor binding protein 1, IGFBP1, AFBP, hIGFBP-1, IBP1, IGF-BP25, PP12, IGF-binding protein 1, Placental protein 12, amniotic fluid binding protein, alpha-pregnancy-associated endometrial globulin

Expression System

HEK293

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its ability to inhibit proliferation using MCF-7 human breast cancer cells in the presence of Human IGF-1. The ED50 range ≤3 µg/mL.

Molecular Weight

25.2kDa (234aa)

Additionnal Information

ATGP4103-10 µg, ATGP4103-20 µg, ATGP4103-50 µg, ATGP4103-100 µg, ATGP4103-250 µg, ATGP4103-500 µg, ATGP4103-1 mg, ATGP4103-10, ATGP4103-20, ATGP4103-50, ATGP4103-100, ATGP4103-250, ATGP4103-500, ATGP4103-1

References & Citations

Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3-34.; ; Kelley, K.M., et al, (1996) Int. J. Biochem. Cell Biol. 28:619-637.; ; J.Ekstrand., et al, (1990) Genomics. 6:413-418.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000587.1

Uniprot Accession Number

P08833

Species

Human

N Terminal Sequence

Ala26

AA Sequence

APWQCAPCSA EKLALCPPVS ASCSEVTRSA GCGCCPMCAL PLGAACGVAT ARCARGLSCR ALPGEQQPLH ALTRGQGACV QESDASAPHA AEAGSPESPE STEITEEELL DNFHLMAPSE EDHSILWDAI STYDGSKALH VTNIKKWKEP CRIELYRVVE SLAKAQETSG EEISKFYLPN CNKNGFYHSR QCETSMDGEA GLCWCVYPWN GKRIPGSPEI RGDPNCQIYF NVQN