Recombinant human CD16a/FCGR3A protein

FCGR3A, also known as low affinity immunoglobulin gamma Fc region receptor III-A, is a multifunctional, low or intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is expressed on human NK cells as an integral membrane glycoprotein anchored through a transmembrane peptide. It mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. Recombinant human FCGR3A, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human Fc gamma RIIIA/CD16a is coated at 10 µg/mL (100 ul/well) can bind Human IgG1 Fc (CAT# ATGP4094) in a functional ELISA assay.

Product Name Alternative

Low affinity immunoglobulin gamma Fc region receptor III-A, low affinity immunoglobulin gamma Fc region receptor III-A isoform c, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, FCGRIII, IGFR3, IMD20, CD16

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human IgG1 Fc (CAT# ATGP4094) .

Molecular Weight

22.8 kDa (200aa)

Additionnal Information

ATGP4102-10 µg, ATGP4102-20 µg, ATGP4102-50 µg, ATGP4102-100 µg, ATGP4102-250 µg, ATGP4102-500 µg, ATGP4102-1 mg, ATGP4102-10, ATGP4102-20, ATGP4102-50, ATGP4102-100, ATGP4102-250, ATGP4102-500, ATGP4102-1, ATGP3449-10 µg, ATGP3449-20 µg, ATGP3449-50 µg, ATGP3449-100 µg, ATGP3449-250 µg, ATGP3449-500 µg, ATGP3449-1 mg, ATGP3449-10, ATGP3449-20, ATGP3449-50, ATGP3449-100, ATGP3449-250, ATGP3449-500, ATGP3449-1

References & Citations

Ravetch JV., et al. (1989) J Exp Med. 170:481-497.; ; Ferrara C., et al. (2011) Proc Natl Acad Sci U S A. 108:12669-12674.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001121065.1

Uniprot Accession Number

P08637

Species

Human

N Terminal Sequence

Ala

AA Sequence

MRTEDLP KAVVFLEPQW YRVLEKDSVT LKCQGAYSPE DNSTQWFHNE SLISSQASSY FIDAATVDDS GEYRCQTNLS TLSDPVQLEV HIGWLLLQAP RWVFKEEDPI HLRCHSWKNT ALHKVTYLQN GKGRKYFHHN SDFYIPKATL KDSGSYFCRG LFGSKNVSSE TVNITITQGL AVSTISSFFP PGYQ