Recombinant human VEGFR1/Flt-1 protein

VEGFR1 (Vascular endothelial growth factor receptor 1), also known as FLT-1 (Fms-like tyrosine kinase 1), is belongs to the class III subfamily of receptor tyrosine kinases. While family members VEGFR1, VEGFR2and VEGFR3 are all mainly expressed on endothelial cells, only VEGFR1 is expressed on macrophages, and mainly plays inhibitory roles. Inhibitors of VEGFR are used in the treatment of cancer. It acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 binds VEGF with higher affinity than does VEGFR2, but shows weaker kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Recombinant human VEGFR1/Flt-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human VEGFR1/Flt-1 inhibit proliferation of the HUVEC human umbilical vein endothelial cells in the presence of Human VEGF165. The ED50 range ≤ 60 ng/mL.

Product Name Alternative

VEGFR1, VEGF R1, VEGFR-1, Vascular endothelial growth factor receptor 1 isoform 1, Fms-like tyrosine kinase 1, FLT-1, FLT1, FLT, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor, fms-related tyrosine kinase 1, vascular endothelial growth factor, vascularpermeability factor receptor

Expression System

HEK293

Antigen Species

Human

Tag

HIgG-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its ability to inhibit proliferation using HUVEC human umbilical vein endothelial cells in the presence of Human VEGF165. The ED50 range ≤ 60 ng/mL.

Molecular Weight

60.3kDa (535aa)

Additionnal Information

ATGP4101-10 µg, ATGP4101-20 µg, ATGP4101-50 µg, ATGP4101-100 µg, ATGP4101-250 µg, ATGP4101-500 µg, ATGP4101-1 mg, ATGP4101-10, ATGP4101-20, ATGP4101-50, ATGP4101-100, ATGP4101-250, ATGP4101-500, ATGP4101-1

References & Citations

Peters, K.G. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8915-8919.; ; Kendall R.L., et al., (1993), Natl. Acad. Sci. U.S.A. 90:10705-10709.; ; Herley M.T., et al., (1999), Biochem. Biophys. Res. Commun. 262:731-738.; ; Kendall R.L., et al., (1993), Natl. Acad. Sci. U.S.A. 90:10705-10709.;