Recombinant human CD16a/FCGR3A protein

Fc gamma RIIIA/CD16a is also known as Low affinity immunoglobulin gamma Fc region receptor III-A isoform c. It is optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC) . Also mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation. Involved in the generation of memory-like adaptive NK cells capable to produce high amounts of IFNG and to efficiently eliminate virus-infected cells via ADCC. Regulates NK cell survival and proliferation, in particular by preventing NK cell progenitor apoptosis. Fc gamma RIIIA is mediates the antitumor activities of therapeutic antibodies. Upon ligation on monocytes triggers TNFA-dependent ADCC of IgG-coated tumor cells. Recombinant human Fc gamma RIIIA/CD16a, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.