Recombinant human FGF-8b protein

FGF-8b, also known as Fibroblast growth factor 8 isoform B, is a member of the fibroblast growth factor (FGF) family that plays a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. There are 4 known alternate spliced forms of FGF-8; FGF-8a, FGF-8b, FGF-8e and FGF-8f. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It encodes a key signaling factor, and its precise regulation is essential for embryo patterning. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity. FGF-8 signaling from the apical ectodermal ridge (AER), which borders the distal end of the limb bud, is necessary for forming normal limbs. FGF-8 is responsible for cell proliferation and survival, as well. The ability for FGF-8 to regulate cell proliferation has caused interest in its effects on tumors or squamous cell carcinoma. Recombinant human FGF-8b, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human FGF-8b stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 µg/mL of heparin. The ED50 range ≤ to 5 µg/mL.

Product Name Alternative

FGF8, AIGF, Androgen-induced growth factor, FGF-8, HBGF-8, Heparin-binding growth factor 8, HH6, KAL6, fibroblast growth factor 8, fibroblast growth factor 8 isoform B

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 µg/mL of heparin. The ED50 range ≤ 5 µg/mL.

Molecular Weight

23.7kDa (204aa)

Additionnal Information

ATGP4097-10 µg, ATGP4097-20 µg, ATGP4097-50 µg, ATGP4097-100 µg, ATGP4097-250 µg, ATGP4097-500 µg, ATGP4097-1 mg, ATGP4097-10, ATGP4097-20, ATGP4097-50, ATGP4097-100, ATGP4097-250, ATGP4097-500, ATGP4097-1

References & Citations

Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928-8932.; ; Ornitz DM, et al. (1996) . The Journal of Biological Chemistry. 271: 15292–15297.; ; Crossley, P.H. et al. (1996) Cell, 84:127-136.; ; Estienne A, et al. (2018) Reproduction. 155:53-62.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.

Scientific Category

Angiogenesis, Cardiovascular

NCBI Accession Number

NP_006110.1

Uniprot Accession Number

P55075

Species

Human

N Terminal Sequence

Asp

AA Sequence

QVTVQ SSPNFTQHVR EQSLVTDQLS RRLIRTYQLY SRTSGKHVQV LANKRINAMA EDGDPFAKLI VETDTFGSRV RVRGAETGLY ICMNKKGKLI AKSNGKGKDC VFTEIVLENN YTALQNAKYE GWYMAFTRKG RPRKGSKTRQ HQREVHFMKR LPRGHHTTEQ SLRFEFLNYP PFTRSLRGSQ RTWAPEPR